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- Title
Biocrystallization in Bacterial and Fungal Cells and Spores.
- Authors
Krupyanskii, Y. F.; Loiko, N. G.; Sinitsyn, D. O.; Tereshkina, K. B.; Tereshkin, E. V.; Frolov, I. A.; Chulichkov, A. L.; Bokareva, D. A.; Mysyakina, I. S.; Nikolaev, Y. A.; El’-Registan, G. I.; Popov, V. O.; Sokolova, O. S.; Shaitan, K. V.; Popov, A. N.
- Abstract
A series of X-ray diffraction experiments were performed for the first time to study stress-induced biocrystallization (structural response to stress) in the bacteria E. coli, the spore-forming bacteria Bacillus cereus, and in cells and spores of the mycelial fungus Umbelopsis ramanniana. High-intensity areas with spacings of 90 and 44 Å are indicative of a periodically ordered arrangement (most likely nanocrystalline) of the bacterial nucleoid. For the starved bacteria Bacillus cereus, a peak at a spacing of 45 Å is also assigned to nanocrystalline complexes of DNA with the Dps protein. The spores of the fungus Umbelopsis ramanniana VKM F-582, as well as the spores of Bacillus cereus, form ordered arrays of DNA molecules with DNA-condensing acid-soluble proteins SASPs. Starved dehydrated mycelial cells of the fungus Umbelopsis ramanniana form ordered structures with spacings from 27 to 55 Å. A series of peaks reflect the formation of a number of ordered protein arrays, apparently with DNA, with continuously varying characteristic interplanar spacings.
- Subjects
BACTERIAL spores; FUNGAL spores; CRYSTALLIZATION; X-ray diffraction; DNA analysis
- Publication
Crystallography Reports, 2018, Vol 63, Issue 4, p594
- ISSN
1063-7745
- Publication type
Article
- DOI
10.1134/S1063774518040144