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- Title
Defeating Huanglongbing Pathogen Candidatus Liberibacter asiaticus With Indigenous Citrus Endophyte Bacillus subtilis L1-21.
- Authors
Munir, Shahzad; Li, Yongmei; He, Pengbo; He, Pengfei; He, Pengjie; Cui, Wenyan; Wu, Yixin; Li, Xingyu; Li, Qi; Zhang, Sixiang; Xiong, Yangsu; Lu, Zhanjun; Wang, Wenbiao; Zong, Kexian; Yang, Yongchao; Yang, Shaocong; Mu, Chan; Wen, Heming; Wang, Yuehu; Guo, Jun
- Abstract
Huanglongbing (HLB) has turned into a devastating botanical pandemic of citrus crops, caused by Candidatus Liberibacter asiaticus (C Las). However, until now the disease has remained incurable with very limited control strategies available. Restoration of the affected microbiomes in the diseased host through the introduction of an indigenous endophyte Bacillus subtilis L1-21 isolated from healthy citrus may provide an innovative approach for disease management. A novel half-leaf method was developed in vitro to test the efficacy of the endophyte L1-21 against C Las. Application of B. subtilis L1-21 at 104 colony forming unit (cfu ml−1) resulted in a 1,000-fold reduction in the C Las copies per gram of leaf midrib (107 to 104) in 4 days. In HLB-affected citrus orchards over a period of 2 years, the C Las incidence was reduced to < 3%, and C Las copies declined from 109 to 104 g−1 of diseased leaf midribs in the endophyte L1-21 treated trees. Reduction in disease incidence may corroborate a direct or an indirect biocontrol effect of the endophytes as red fluorescent protein-labeled B. subtilis L1-21 colonized and shared niche (phloem) with C Las. This is the first large-scale study for establishing a sustainable HLB control strategy through citrus endophytic microbiome restructuring using an indigenous endophyte.
- Subjects
CANDIDATUS liberibacter asiaticus; CITRUS; BACILLUS subtilis; DISEASE incidence; DISEASE management; PATHOGENIC microorganisms
- Publication
Frontiers in Plant Science, 2022, Vol 13, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2022.884890