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- Title
Two-Dimensional Fluorescence Difference Spectroscopy of ZnO and Mg Composites in the Detection of Physiological Protein and RNA Interactions.
- Authors
Hoffman, Amanda; Xiaotong Wu; Jianjie Wang; Brodeur, Amanda; Thomas, Rintu; Thakkar, Ravindra; Hadi, Halena; Glaspell, Garry P.; Duszynski, Molly; Wanekaya, Adam; DeLong, Robert K.
- Abstract
Two-dimensional fluorescence difference spectroscopy (2-D FDS) was used to determine the unique spectral signatures of zinc oxide (ZnO), magnesium oxide (MgO) and 5% magnesium zinc oxide nanocomposite (5% Mg/ZnO) and was then used to demonstrate the change in spectral signature that occurs when physiologically important proteins, such as angiotensin-converting enzyme (ACE) and ribonuclease A (RNase A), interact with ZnO nanoparticles (NPs). When RNase A is bound to 5% Mg/ZnO, the intensity is quenched, while the intensity is magnified and a significant shift is seen when torula yeast RNA (TYRNA) is bound to RNase A and 5% Mg/ZnO. The intensity of 5% Mg/ZnO is quenched also when thrombin and thrombin aptamer are bound to the nanocomposite. These data indicate that RNA-protein interaction can occur unimpeded on the surface of NPs, which was confirmed by gel electrophoresis and importantly that the change in fluorescence excitation, emission and intensity shown by 2-D FDS may indicate specificity of biomolecular interactions.
- Subjects
ZINC oxide spectra; FLUORESCENCE spectroscopy; BIOSENSORS; PROTEIN analysis; RNA analysis; FLUORIMETRY
- Publication
Materials (1996-1944), 2017, Vol 10, Issue 12, p1430
- ISSN
1996-1944
- Publication type
Article
- DOI
10.3390/ma10121430