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- Title
Interference of cyclosporin with lymphocyte activation: blockage of the mitogen-induced increases of lysosomal and mitochondrial activities.
- Authors
Koponen, M.; Grieder, A.; Loort, F.
- Abstract
Mouse lymphocytes were activated by a mitogenic dose of concanavalin A and analysed by flow cytometry to monitor the increases of mitochondrial activity (using rhodamine 123 ns probe) and of lysosomal activity (using euchrysin [acridine orange] as probe). Cyclosporin A-treated lymphocytes were not capable of responding to concanavalin A in the same way as untreated lymphocytes: both the increased uptakes of rhodamine 123 by mitochondria and of acridine orange by lysosomes were strongly diminished, though not abolished. Cyclosporin may thus interfere at a step of activation prior or concurrent to those early changes of lymphocyte physiology. It looks like that it allows mitogen-activated cells to go through part of the mitochondrial maturation which precedes initiation of nuclear DNA synthesis, after which the cells remain blocked at that incomplete maturation level.
- Subjects
CYCLOSPORINE; IMMUNOSUPPRESSIVE agents; CYCLIC peptides; MITOGENS; MITOCHONDRIA; ORGANELLES
- Publication
Immunology, 1984, Vol 53, Issue 1, p55
- ISSN
0019-2805
- Publication type
Article