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- Title
Nutrient control of eukaryote cell growth: asystems biology study in yeast.
- Authors
Gutteridge, Alex; Pir, Pinar; Castrillo, Juan I.; Charles, Philip D.; Lilley, Kathryn S.; Oliver, Stephen G.
- Abstract
Background: To elucidate the biological processes affected by changes in growth rate and nutrient availability, we have performed a comprehensive analysis of the transcriptome, proteome and metabolome responses of chemostat cultures of the yeast, Saccharomyces cerevisiae, growing at a range of growth rates and in four different nutrient-limiting conditions. Results: We find significant changes in expression for many genes in each of the four nutrient-limited conditions tested. We also observe several processes that respond differently to changes in growth rate and are specific to each nutrient-limiting condition. These include carbohydrate storage, mitochondrial function, ribosome synthesis, and phosphate transport. Integrating transcriptome data with proteome measurements allows us to identify previously unrecognized examples of post-transcriptional regulation in response to both nutrient and growth-rate signals. Conclusions: Our results emphasize the unique properties of carbon metabolism and the carbon substrate, the limitation of which induces significant changes in gene regulation at the transcriptional and post-transcriptional level, as well as altering how many genes respond to growth rate. By comparison, the responses to growth limitation by other nutrients involve a smaller set of genes that participate in specific pathways. See associated commentary http://www.biomedcentral.com/1741-7007/8/62
- Subjects
EUKARYOTIC cells; GENETIC regulation; SYSTEMS biology; YEAST; PLANT nutrients
- Publication
BMC Biology, 2010, Vol 8, p68
- ISSN
1741-7007
- Publication type
Article
- DOI
10.1186/1741-7007-8-68