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- Title
Nucleosome positioning at the replication fork.
- Authors
Lucchini, Renzo; Wellinger, Raif Erik; Sogo, José M.
- Abstract
In order to determine the time required for nucleosomes assembled on the daughter strands of replication forks to assume favored positions with respect to DNA sequence, psoralen cross-linked replication intermediates purified from preparative two-dimensional agarose gets were analyzed by exonuclease digestion or primer extension. Analysis of sites of psoralen intercalation revealed that nucleosomes in the yeast Saccharomyces cerevisiae rDNA intergenic spacer are positioned shortly after passage of the replication machinery. Therefore, both the 'old' randomly segregated nucleosomes as welt as the 'new' assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands, suggesting that the positioning of nucleosomes is an initial step in the chromatin maturation process.
- Subjects
SACCHAROMYCES; RECOMBINANT DNA; DNA replication; PSORALENS; DNA probes; EXONUCLEASES
- Publication
EMBO Journal, 2001, Vol 20, Issue 24, p7294
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/20.24.7294