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- Title
Lovastatin biosynthetic genes of Aspergillus terreus are expressed differentially in solid-state and in liquid submerged fermentation.
- Authors
Barrios-González, J.; Baños, J. G.; Covarrubias, A. A.; Garay-Arroyo, A.
- Abstract
Molecular studies were performed to establish the causes of the superior lovastatin productivity of a novel solid-state fermentation (SSF) process, in relation with liquid submerged fermentation (SmF; 20 mg/g vs. 0.65 mg/ml). In SSF, biosynthetic genes lovE and lovF transcripts accumulated to high levels from day 1 to day 7. In this period, lovE transcript showed 4.6-fold higher accumulation levels (transcription) than the highest level detected in SmF (day 5). lovF transcript showed two-fold higher expression than the highest point in SmF. In SmF, the expression was only detected clearly on day 5 and, showing a 50% decrease, on day 7. These results show that the higher lovastatin production in SSF is related to a more intense transcription of these biosynthetic genes. A strong expression of gldB gene in lovastatin SSF indicated that Aspergillus terreus senses osmotic stress during the course of SSF, but not in SmF. However, when a liquid medium of identical concentration was used in SmF, lovastatin production decreased in 50%, while lovE and lovF transcripts accumulation was 20 and six-fold lower than in SSF; showing that physiology is different in SSF, and that osmotic stress alone is not responsible for the higher gene expression in SSF.
- Subjects
BIOSYNTHESIS; BIOCHEMICAL engineering; GENES; ASPERGILLUS; GENE expression; GENETIC regulation; FERMENTATION; MICROBIOLOGICAL synthesis; BIOTECHNOLOGY
- Publication
Applied Microbiology & Biotechnology, 2008, Vol 79, Issue 2, p179
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-008-1409-2