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- Title
Involvement of mitogen-activated protein kinases and nuclear factor kappa B pathways in signaling COX-2 expression in chronic rhinosinusitis.
- Authors
Zhenlin Wang; Qiuhang Zhang; Yuan Li; Peng Li; Gehua Zhang; Yulu Li
- Abstract
Abstract Objective To investigate the signal pathways involved in cyclooxygenase-2 (COX-2) expression in chronic rhinosinusitis (CRS). Methods The expressions of COX-2, p38 mitogen-activated protein kinase (p38MAPK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa B (NF-κB) in nasal mucosa were detected by immunohistological stain and polymerase chain reaction (PCR). Their expressions and prostaglandin E2 (PGE2) release were determined by PCR, Western blot and enzyme immunoassay (EIA) in human nasal epithelia (HNE) cells after lipopolysaccharide (LPS) induction, and/or small interfering RNA (siRNA) transfection. Results Positive protein expressions of COX-2, p38MAPK, ERK, NF-κB subunits were detected in epithelial and inflammatory cells. Their mRNA levels were significantly higher in CRS than controls (P Conclusion Upregulation of COX-2 expression suggested its role as a mediator in CRS. ERK and p38MAPK pathways were involved in signaling COX-2 through NF-κB pathway.
- Subjects
MITOGEN-activated protein kinases; NF-kappa B; CELLULAR signal transduction; CYCLOOXYGENASE 2; CHRONIC diseases; SINUSITIS; GENE expression; IMMUNOHISTOCHEMISTRY
- Publication
Inflammation Research, 2009, Vol 58, Issue 10, p649
- ISSN
1023-3830
- Publication type
Article
- DOI
10.1007/s00011-009-0030-x