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- Title
nAflatoxin determination in Saffron by high-performance liquid chromatography and immunoaffinity column cleanup.
- Authors
Hamed Reza Beheshti; Feizi, Javad; Mahnaz Zhiany Asgharzadeh; Somayeh Sadat Fakoor Janati
- Abstract
In this paper, method based on high performance liquid chromatography with fluorescence detection has been suggested to measure aflatoxin in saffron. This method required a simple extraction of aflatoxin using MeOH/H2O (80:20, v/v) and a purification by immunoaffinity column cleanup. Aflatoxin measurement was performed at an emission wavelength of 445 nm and an excitation wavelength of 365 nm. Detection limits for AFB1, AFB2, AFG1 and AFG2 were 0.293, 0.08, 0.55, and 0.30 ng g-1, respectively. The percentage of Relative standard deviations for measuring aflatoxin is in the range of 1.33-5.10 % and the percentage of recovery is in the range of 94-67. Regarding The overall results of high-performance liquid chromatography applied in this experiment, we can realize that this method can be used for detection and measurement of different kinds of aflatoxins in saffron.
- Subjects
LIQUID chromatography; FLUORESCENCE; AFLATOXINS; IMMUNOAFFINITY chromatography; STANDARD deviations
- Publication
Saffron Agronomy & Technology, 2016, Vol 4, p34
- ISSN
2383-1529
- Publication type
Article
- DOI
10.22048/jsat.2014.4820