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- Title
Molecular modeling of the HAMP domain of sensory rhodopsin II transducer from Natronomonas pharaonis.
- Authors
Nishikata, Koro; Fuchigami, Sotaro; Ikeguchi, Mitsunori; Kidera, Akinori
- Abstract
The halobacterial transducer of sensory rhodopsin II (HtrII) is a photosignal transducer associated with phototaxis in extreme halophiles. The HAMP domain, a linker domain in HtrII, is considered to play an important role in transferring the signal from the membrane to the cytoplasmic region, although its structure in the complex remains undetermined. To establish the structural basis for understanding the mechanism of signal transduction, we present an atomic model of the structure of the Nterminal HAMP domain from Natronomonas pharaonis (HtrII: 84-136), based on molecular dynamics (MD) simulations. The model was built by homology modeling using the NMR structure of Af1503 from Archaeoglobus fulgidus as a template. The HAMP domains of Af1503 and HtrII were stable during MD simulations over 100 ns. Quantitative analyses of inter-helical packing indicated that the Af1503 HAMP domain stably maintained unusual knobs-to-knobs packing, as observed in the NMR structure, while the bulky side-chains of HtrII shifted the packing state to canonical knobs-into-holes. The role of the connector loop in maintaining structural stability was also discussed using MD simulations of loop deletion mutants.
- Subjects
MOLECULAR dynamics; MOLECULAR beams; CELLULAR signal transduction; PHYSIOLOGICAL control systems; BIOENERGETICS; CELLULAR control mechanisms
- Publication
Biophysics (13492942), 2010, Vol 6, p27
- ISSN
1349-2942
- Publication type
Article
- DOI
10.2142/biophysics.6.27