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- Title
Evaluation of dendrite morphology in Wistar and genetic absence epileptic (GAERS) rats.
- Authors
Yazi, Sevdenur; Gülhan, Rezzan; Onat, Filiz; Şehirli, Ümit S.; Kirazlı, Özlem
- Abstract
Objective: The aim of the study is to examine the morphological features of dendrites and dendritic spines of pyramidal neurons in somatosensory cortex and hippocampus of Wistar and GAERS (Genetic Absence Epilepsy Rat from Strasbourg) rats. Methods: GAERS (n=5) and Wistar (n=5) rats were sacrificed by transcardial perfusion method. Brain tissues were stained using the FD Rapid GolgiStain Kit. Coronal sections of 200 μm thickness were obtained with cryostat. Pyramidal neurons in deep layers of the somatosensory cortex and CA1 region of the hippocampus were examined using a light microscope and Neurolucida 360 software. Branching and length of apical dendrite and basal dendrites, and types and densities of dendritic spines were analyzed. Results: Statistically the total number of dendrite nodes (p=0.0053, p=0.0047), the total number of dendrite segments (dendritic arborization) (p=0.0036, p=0.0036), the total number of dendrite terminations (p=0.0033, p=0.0029), the total dendrite length (μm) (p=0.0002, p=0.0007) and the dendritic spine density (1/μm) (p=0.0168, p=0.0120) of the somatosensory cortex and the hippocampus were significantly higher in GAERS rats, respectively. When dendritic spine types were evaluated separately, stubby type dendritic spines in the hippocampus were found to be significantly higher in GAERS rats compared to Wistar rats (p=0.0204). Conclusion: It was concluded that intense synaptic activation seen in postsynaptic cells in the somatosensory cortex and hippocampus in GAERS causes changes in the dendrite morphology of pyramidal neurons. Grant declaration: This study was supported by Marmara University Scientific Research Projects Commission (TYL-2021-10244).
- Subjects
STRASBOURG (France); DENDRITIC spines; DENDRITES; PYRAMIDAL neurons; SOMATOSENSORY cortex; MORPHOLOGY; LABORATORY rats; RATS; MICROSCOPES
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2022, Vol 16, pS131
- ISSN
1307-8798
- Publication type
Article