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- Title
ANALYTICAL AND CLINICAL EVALUATION OF SYSMEX UF1000I FOR AUTOMATED SCREENING OF CEREBROSPINAL FLUIDS ANALITIČKA I KLINIČKA EVALUACIJA UREĐAJA SYSMEX UF1000I ZA AUTOMATSKI SKRINING CEREBROSPINALNIH TEČNOSTI.
- Authors
Buoro, Sabrina; Ottomano, Cosimo; Esposito, Sara Appassiti; Gherardi, Patrizia; Alessio, Maria Grazia; Crippa, Alberto; Raglio, Claudio FarinaAnnibale; Lippi, Giuseppe
- Abstract
Background: We evaluated the performance of Sysmex UF- 1000i for cell counting and differential cell count, as well as for assessment of bacteria load in cerebrospinal fluid (CSF), as a potential approach for the rapid screening of meningitis or bacterial encephalitis. Methods: We analyzed 77 consecutive CSF samples, 34 of which (44%) displayed leukocyte count >5 white blood cell (WBQ/^iL with optical microscopy. Results on the UF-1000i were compared with those obtained by microscopic analysis. Imprecision was evaluated by testing three CSF samples with leukocyte values between 3.5 and 28.8 WBC/nL in 10 repli- cates. Carry-over was evaluated with the Broughton formula on three CSF pools with leukocyte counts between 93.5 and 132.5 WBC/^L. Linearity was assessed according to CLSI document EP6-A. In the presence of bacteria, identification and antibiogram were performed with Vitex (Biomerieux), except for Neisserie meningitidis (ApiNH, Biomerieux). Sensitivity tests were performed with Vitex and disc diffusion. Results: Optimal correlation was found between UF-1000i and optical microscopy, displaying Pearson's correlation of 0.99 and mean bias of-3.5 WBC/^L (95% Cl, from -7.0 to 0.0 WBC/nL). Imprecision varied between 12 and 16%. Li- nearity was excellent, 4-278 WBC/nL. Carry-over was neg- ligible. ROC analysis yielded AUC of 0.99 for both WBC and bacterial counts. The agreement at threshold >4 WBC/nL was 0.91, with sensitivity and specificity of 1.00 and 0.84. At S19 bacteria^nL cut-off, accuracy was 0.98, sensitivity 1.00 and specificity 0.97. Conclusions: According to these results, CSF screening with UF-I000i seems a reliable approach in terms of instrument performance, turnaround time and overall laboratory effi- ciency.
- Subjects
CEREBROSPINAL fluid; ENCEPHALITIS diagnosis; MENINGITIS diagnosis; LEUKOCYTE count; CYTOFLUOROMETRY
- Publication
Journal of Medical Biochemistry, 2014, Vol 33, Issue 2, p191
- ISSN
1452-8258
- Publication type
Article
- DOI
10.2478/jomb-2013-0034