We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Differential gene expression signatures for cell wall integrity found in chitin synthase 11 (chs2∆) and myosin 11 (myol∆) deficient cytokinesis mutants of Saccharomyces cerevisiae.
- Authors
Rodríguez-Quiñones, José F.; Rodríguez-Medina, José R.
- Abstract
Background: Myosin II-dependent contraction of the cytokinetic ring and primary septum formation by chitin synthase II are interdependent processes during cytokinesis in Saccharomyces cerevisiae. Hence, null mutants of myosin II (myo1Δ) and chitin synthase II (chs2Δ) share multiple morphological and molecular phenotypes. To understand the nature of their interdependent functions, we will seek to identify genes undergoing transcriptional regulation in chs2Δ strains and to establish a transcription signature profile for comparison with myo1Δ strains. Results: A total of 467 genes were commonly regulated between myo1 Δ and chs2 Δ mutant strains (p ⩽ 0.01). Common regulated biological process categories identified by Gene Set Enrichment Analysis (GSEA) in both gene expression profiles were: protein biosynthesis, RNA processing, and stress response. Expression of 17/20 genes in the main transcriptional fingerprint for cell wall stress was confirmed in the chs2 Δ strain versus 5/20 for the myo1 Δ strain. One of these genes, SLT2/ MPK1, was up-regulated in both strains and both strains accumulated the hyperphosphorylated form of Slt2p thereby confirming that the PKC1 cell wall integrity pathway (CWIP) was activated by both mutations. The SLT2/MPK1 gene, essential for myo1 Δ strains, was not required in the chs2 Δ strain. Conclusion: Comparison of the chs2 Δ and myo1Δ gene expression profiles revealed similarities in the biological process categories that respond to the chs2 Δ and myo1Δ gene mutations. This supports the view that these mutations affect a common function in cytokinesis. Despite their similarities, these mutants exhibited significant differences in expression of the main transcriptional fingerprint for cell wall stress and their requirement of the CWIP for survival.
- Subjects
GENE expression; GENETIC mutation; GENETIC regulation; POLYSACCHARIDES; GENES; ANTHROPOMETRY; BIOCHEMISTRY; PROTEIN synthesis; RNA
- Publication
BMC Research Notes, 2009, Vol 2, p1
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/1756-0500-2-87