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- Title
The inhibitory effect of ketotifen on entrance of Toxoplasma gondii tachyzoites into macrophages of mouse.
- Authors
Daryani, Ahmad; Ebrahimzadeh, Mohammad-Ali; Sharif, Mehdi; Rezaei, Fatemeh; Ahmadpour, Ehsan; Sarvi, Shahab; Ajami, Abolghasem; Ziaei, Hajar; Khalilian, Alireza
- Abstract
Background and purpose: Toxoplasma gondii is a protozoon with worldwide distribution. In spite of increasing information about its biology, treatment of toxoplasmosis is restricted to a few drugs and unfortunately using of each of drugs is associated with significant side effects in patients. It seems that investigation on alternative drugs is necessary. Materials and methods: In case group, concentrations of 1, 5 and 10 μg/ml of ketotifen were prepared and added to RPMI (Roswell Park Memorial Institute) medium containing peritoneal macrophages. After 60 minutes, incubation and adding Toxoplasma gondii tachyzoites to medium, the inhibitory rate of different concentrations of ketotifen on entrance of Toxoplasma tachyzoites into macrophages in 30 and 60 minutes were evaluated. Control group did not receive ketotifen. Results: The best efficacy of ketotifen in inhibition of entrance of Toxoplasma tachyzoites into macrophages was observed in 10 μg/ml and in 60 minutes (72.90 ± 1.70) (P < 0.05). Conclusion: The findings of this research show that ketotifen is a suitable drug for inhibiting the entrance of Toxoplasma gondii tachyzoites into nucleated cells in vitro.
- Subjects
TOXOPLASMA gondii; KETOTIFEN; MACROPHAGES; LABORATORY mice; TOXOPLASMOSIS treatment; PHARMACEUTICAL research
- Publication
Journal of Mazandaran University of Medical Sciences (JMUMS), 2014, Vol 23, Issue 110, p74
- ISSN
1735-9260
- Publication type
Article