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- Title
EXPERIMENTAL INFECTION OF PEROMYSCUS CALIFORNICUS WITH TOXOPLASMA GONDII.
- Authors
Dabritz, Haydee A.; Miller, Melissa A.; Packham, Andrea E.; Rejmanek, Daniel; Leutenegger, Christian M.; Gardner, Ian A.; Atwill, E. Robert; Conrad, Patricia A.
- Abstract
Eight female Peromyscus californicus were infected with 102 or 104 Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 104 tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.
- Subjects
SCIENTIFIC experimentation; PEROMYSCUS; TOXOPLASMA gondii; PARASITISM; HOST-parasite relationships; IMMUNOHISTOCHEMISTRY; POLYMERASE chain reaction; LABORATORY mice
- Publication
Journal of Parasitology, 2007, Vol 93, Issue 6, p1360
- ISSN
0022-3395
- Publication type
Article
- DOI
10.1645/GE-1210.1