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- Title
Evaluation of respiratory syncytial virus group A and B genotypes among nosocomial and community-acquired pediatric infections in southern Brazil.
- Authors
de-Paris, Fernanda; Beck, Caroline; de Souza Nunes, Luciana; Mombach Pinheiro Machado, Alice Beatriz; Minuto Paiva, Rodrigo; da Silva Menezes, Denise; Rosane Pires, Márcia; Pires dos Santos, Rodrigo; de Souza Kuchenbecker, Ricardo; Barth, Afonso Luis
- Abstract
Background Respiratory syncytial virus (RSV) is the main cause of lower respiratory tract illness in children worldwide. Molecular analyses show two distinct RSV groups (A and B) that comprise different genotypes. This variability contributes to the capacity of RSV to cause yearly outbreaks. These RSV genotypes circulate within the community and within hospital wards. RSV is currently the leading cause of nosocomial respiratory tract infections in pediatric populations. The aim of this study was to evaluate the G protein gene diversity of RSV amplicons. Methods Nasopharyngeal aspirate samples were collected from children with nosocomial or community-acquired infections. Sixty-three RSV samples (21 nosocomial and 42 community-acquired) were evaluated and classified as RSV-A or RSV-B by real-time PCR. Sequencing of the second variable region of the G protein gene was performed to establish RSV phylogenetics. Results We observed co-circulation of RSV-A and RSV-B, with RSV-A as the predominant group. All nosocomial and community-acquired RSV-A samples were from the same phylogenetic group, comprising the NA1 genotype, and all RSV-B samples (nosocomial and communityacquired) were of the BA4 genotype. Therefore, in both RSV groups (nosocomial and community-acquired), the isolates belonged to only one genotype in circulation. Conclusions This is the first study to describe circulation of the NA1 RSV genotype in Brazil. Furthermore, this study showed that the BA4 genotype remains in circulation. Deciphering worldwide RSV genetic variability will aid vaccine design and development.
- Subjects
BRAZIL; RESPIRATORY syncytial virus genetics; NOSOCOMIAL infections; HOSPITALS; HUMAN genetic variation; G protein genetics
- Publication
Virology Journal, 2014, Vol 11, Issue 1, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/1743-422X-11-36