We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Reduced Levels of Circulating 7a-Hydroxy-Dehydroepiandrosterone in Treated Adolescent Obese Patients.
- Authors
MÁČOVÁ, L.; BIČÍKOVÁ, M.; ZAMRAZILOVÁ, H.; HILL, M.; KAZIHNITKOVÁ, H.; SEDLÁCKOVÁ, B.; STÁRKA, L.
- Abstract
Elevated levels of glucocorticoids lead to the development of obesity and metabolic syndrome. Local glucocorticoid levels are regulated through the enzyme 11ß-hydroxysteroid dehydrogenase 1 (11ß-HSD 1), an enzyme that regenerates active cortisol from inert cortisone. Increased expression of 11ß- HSD 1 in adipose tissue promotes higher body mass index (BMI), insulin resistance, hypertension, and dyslipidemia. Human 11ß- HSD 1 is also responsible for inter-conversion of 7-hydroxylate metabolites of dehydroepiandrosterone (7-OH-DHEA) to their 7- oxo-form. To better understanding the mechanism of the action, we focused on 7-OH- and 7-oxo-DHEA, and their circulating levels during the reductive treatment in adolescent obese patients. We determined plasma levels of 7a-OH-DHEA, 7ß-OHDHEA, and 7-oxo-DHEA in 55 adolescent patients aged 13.04- 15.67 years, BMI greater than 90th percentile. Samples were collected before and after one month of reductive therapy. Circulating levels of 7a-OH-DHEA decreased during the reductive therapy from 1.727 (1.614; 1.854, transformed mean with 95 % confidence interval) to 1.530 nmol/l (1.435; 1.637, p<0.05) in girls and from 1.704 (1.583; 1.842) to 1.540 nmol/l (1.435; 1.659, p<0.05) in boys. With regard to the level of 7-oxo-DHEA, a significant reduction from 1.132 (1.044; 1.231) to 0.918 nmol/l (0.844; 1.000, p<0.05) was found after the treatment, but only in boys. No significant difference in 7ß-OH-DHEA levels was observed. In conclusions, diminished levels of 7a-OH-DHEA indicate its possible effect on activity of 11ß-HSD 1. Further studies are necessary to clarify whether competitive substrates for 11ß-HSD 1 such as 7a-OH-DHEA could inhibit production of glucocorticoids and may be involved in metabolic processes leading to reduction of obesity.
- Subjects
OBESITY treatment; DEHYDROEPIANDROSTERONE; GLUCOCORTICOIDS; METABOLIC syndrome; HYDROXYSTEROID dehydrogenases; GENE expression; INSULIN resistance
- Publication
Physiological Research, 2014, Vol 63, Issue 1, p95
- ISSN
0862-8408
- Publication type
Article
- DOI
10.33549/physiolres.932540