We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Allele-selective inhibition of ataxin-3 (ATX3) expression by antisense oligomers and duplex RNAs.
- Authors
Hu, Jiaxin; Gagnon, Keith T.; Liu, Jing; Watts, Jonathan K.; Syeda-Nawaz, Jeja; Bennett, C. Frank; Swayze, Eric E.; Randolph, John; Chattopadhyaya, Jyoti; Corey, David R.
- Abstract
Spinocerebellar ataxia-3 (also known as Machado-Joseph disease) is an incurable neurodegenerative disorder caused by expression of a mutant variant of ataxin-3 (ATX3) protein. Inhibiting expression of ATX3 would provide a therapeutic strategy, but indiscriminant inhibition of both wild-type and mutant ATX3 might lead to undesirable side effects. An ideal silencing agent would block expression of mutant ATX3 while leaving expression of wild-type ATX3 intact. We have previously observed that peptide nucleic acid (PNA) conjugates targeting the expanded CAG repeat within ATX3 mRNA block expression of both alleles. We have now identified additional PNAs capable of inhibiting ATX3 expression that vary in length and in the nature of the conjugated cation chain. We can also achieve potent and selective inhibition using duplex RNAs containing one or more mismatches relative to the CAG repeat. Anti-CAG antisense bridged nucleic acid oligonucleotides that lack a cationic domain are potent inhibitors but are not allele-selective. Allele-selective inhibitors of ATX3 expression provide insights into the mechanism of selectivity and promising lead compounds for further development and in vivo investigation.
- Subjects
GENE frequency; ANTISENSE RNA; OLIGOMERS; FRIEDREICH'S ataxia; GENE expression; NUCLEIC acids; CATIONS; SMALL interfering RNA
- Publication
Biological Chemistry, 2011, Vol 392, Issue 4, p315
- ISSN
1431-6730
- Publication type
Article
- DOI
10.1515/BC.2011.045