We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Gelsolin Inhibits the Inflammatory Process Induced by LPS.
- Authors
Cheng, Ying; Hu, Xuguang; Liu, Cong; Chen, Ming; Wang, Jianing; Wang, Mingyu; Gao, Fu; Han, Jiaqi; Zhang, Chaoxiong; Sun, Ding; Min, Rui
- Abstract
Background/Aims: Endotoxemia is a life-threatening situation that signifies a key challenge in the field of intensive care medicine. Proinflammatory mediators produced by macrophages play a key role in endotoxemia. Gelsolin (GSN) is involved in the process of inflammation. Methods: IL-6 and TNF-α in the supernatant were measured with an ELISA kit. NO production was assessed by measurement of nitrite concentration with the Griess assay. si-RNA directed against GSN (si-GSN) was transfected by Lipofectamine. Results: LPS decreased the levels of GSN. Recombinant GSN inhibited the cytokines induced by LPS and rescued mice from LPSinduced death, and si-GSN increased death in the LPS-pretreated mice. Conclusion: GSN exhibited a protective role in endotoxemia.
- Subjects
GELSOLIN; ENDOTOXEMIA; TUMOR necrosis factors; INTERLEUKIN-6; LIPOPOLYSACCHARIDES
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2017, Vol 41, Issue 1, p205
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000456043