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- Title
GNM C7-8 实时荧光PCR 同时快速检测8 种动物源性成分.
- Authors
胡连霞; 项佳林; 张亦琴; 付 琦; 陈敏娜; 丁红田; 苗 丽; 孙晓霞; 王建昌; 郭春海
- Abstract
Objective To establish a method for simultaneous rapid detection of 8 kinds of animal-derived ingredients by GNM C7-8 real-time PCR system with 8 modules. Methods Based on porcine prion gene primers, bovine growth hormone gene, ovine growth hormone gene, donkey mitochondrial ATPase 6 gene, horse mitochondrial ATPase 6 gene, duck growth hormone gene, mink mitochondrial cytochrome b gene, fox mitochondrial cytochrome oxidase I subunit gene, 8 kinds of real-time PCR detection methods of animal derived components were designed. GNM C7-8 real-time fluorescence PCR system was used to initiate simultaneous detection of 8 animal-derived components and sequential initiation of different time detection at 5 min intervals, and the results were compared with those of ABI 7500 fluorescent PCR. Results GNM C7-8 real-time fluorescence PCR system was used for the simultaneously detection of 8 animal origin components of pig, cow, sheep, donkey, horse, duck, mink and fox, and the number of peak cycles was 30, 27, 21, 21, 24, 17, 15, 17, respectively, which were the same as the detection results at different times. Compared with the detection results of ABI 7500 fluorescence PCR system, the peaking of the 5 components of pig, sheep, horse, duck and mink was 1~2 cycles faster, the detection results of the other 3 components were consistent, and the detection completion time was 16~20 min less. Conclusions The GNM C7-8 real-time fluorescent PCR system operates independently of each other, without interference, without cross-contamination, and can quickly detect 8 animal-derived components at the same time, which provides powerful equipment technical support for rapid screening of animal-derived components.
- Publication
Journal of Food Safety & Quality, 2019, Vol 10, Issue 15, p5123
- ISSN
2095-0381
- Publication type
Article