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- Title
酿酒酵母表面展示金属硫蛋白吸附葡萄酒中的铜离子.
- Authors
黄蓉; 吕林灿; 李莹; 张阳; 刘延琳; 宋育阳
- Abstract
During the winemaking process, excessive copper has the effect of inhibiting fermentation} causing oxidative browning of wine. Metallothionein is a type of protein with high cysteine content and low molecular weight that can be induced by metals. The use of cell surface display technology to express that metallothionein can largely avoid the problem of metal ions transmembrane and reduce the damage of heavy metals to cells. In this study, the metallothionein gene CUP1 from Saccharomyces cerevisiae 288c was displayed on the surface of S・ cerevisiae cells} and a diploid strain with higher adsorption capacity for copper ions in wine was successfully obtained. The research can provide new ideas for solving the problem of copper in wine and lay a theoretical foundation for the industrial application of CUP1 metallothionein whole-cell adsorbent.
- Subjects
COPPER ions; SURFACES (Technology); SACCHAROMYCES cerevisiae; MOLECULAR weights; ADSORPTION capacity
- Publication
Journal of Chinese Institute of Food Science & Technology, 2022, Vol 22, Issue 7, p31
- ISSN
1009-7848
- Publication type
Article
- DOI
10.16429/j.1009-7848.2022.07.003