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- Title
Cell-autonomous regulation of hematopoietic stem cell cycling activity by ATP.
- Authors
Casati, A.; Frascoli, M.; Traggiai, E.; Proietti, M.; Schenk, U.; Grassi, F.
- Abstract
Extracellular nucleotides regulate many cellular functions through activation of purinergic receptors in the plasma membrane. Here, we show that in hematopoietic stem cell (HSC), ATP is stored in vesicles and released in a calcium-sensitive manner. HSC expresses ATP responsive P2X receptors and in vitro pharmacological P2X antagonism restrained hematopoietic progenitors proliferation, but not myeloid differentiation. In mice suffering from chronic inflammation, HSCs were significantly expanded and their cycling activity was sensitive to treatment with the P2X antagonist periodate-oxidized 2,3-dialdehyde ATP. Our results indicate that ATP acts as an autocrine stimulus in regulating HSCs pool size.
- Subjects
HEMATOPOIETIC stem cells; ADENOSINE triphosphate; CELL cycle; NUCLEOTIDES; CELL membranes; CELL metabolism
- Publication
Cell Death & Differentiation, 2011, Vol 18, Issue 3, p396
- ISSN
1350-9047
- Publication type
Article
- DOI
10.1038/cdd.2010.107