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- Title
Genomic programming of IRF4-expressing human Langerhans cells.
- Authors
Sirvent, Sofia; Vallejo, Andres F.; Davies, James; Clayton, Kalum; Wu, Zhiguo; Woo, Jeongmin; Riddell, Jeremy; Chaudhri, Virendra K.; Stumpf, Patrick; Nazlamova, Liliya Angelova; Wheway, Gabrielle; Rose-Zerilli, Matthew; West, Jonathan; Pujato, Mario; Chen, Xiaoting; Woelk, Christopher H.; MacArthur, Ben; Ardern-Jones, Michael; Friedmann, Peter S.; Weirauch, Matthew T.
- Abstract
Langerhans cells (LC) can prime tolerogenic as well as immunogenic responses in skin, but the genomic states and transcription factors (TF) regulating these context-specific responses are unclear. Bulk and single-cell transcriptional profiling demonstrates that human migratory LCs are robustly programmed for MHC-I and MHC-II antigen presentation. Chromatin analysis reveals enrichment of ETS-IRF and AP1-IRF composite regulatory elements in antigen-presentation genes, coinciding with expression of the TFs, PU.1, IRF4 and BATF3 but not IRF8. Migration of LCs from the epidermis is accompanied by upregulation of IRF4, antigen processing components and co-stimulatory molecules. TNF stimulation augments LC cross-presentation while attenuating IRF4 expression. CRISPR-mediated editing reveals IRF4 to positively regulate the LC activation programme, but repress NF2EL2 and NF-kB pathway genes that promote responsiveness to oxidative stress and inflammatory cytokines. Thus, IRF4-dependent genomic programming of human migratory LCs appears to enable LC maturation while attenuating excessive inflammatory and immunogenic responses in the epidermis. Langerhans cells (LC) can prime tolerogenic as well as immunogenic responses in the skin. Here the authors show, by transcriptomic, epigenetic and CRISPR editing analyses, that during LC migration and maturation the transcription factor IRF4 regulates expression of antigen presentation and co-stimulatory gene modules while attenuating inflammatory response genes.
- Subjects
LANGERHANS cells; ANTIGEN presentation; GENE regulatory networks; ANTIGEN processing; TRANSCRIPTION factors; PLURIPOTENT stem cells; ANTIGEN presenting cells
- Publication
Nature Communications, 2020, Vol 11, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-019-14125-x