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- Title
Tomato fruits expressing a bacterial feedback-insensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase of the shikimate pathway possess enhanced levels of multiple specialized metabolites and upgraded aroma.
- Authors
Tzin, Vered; Rogachev, Ilana; Meir, Sagit; Moyal Ben Zvi, Michal; Masci, Tania; Vainstein, Alexander; Aharoni, Asaph; Galili, Gad
- Abstract
Tomato (Solanum lycopersicum) fruit contains significant amounts of bioactive compounds, particularly multiple classes of specialized metabolites. Enhancing the synthesis and accumulation of these substances, specifically in fruits, are central for improving tomato fruit quality (e.g. flavour and aroma) and could aid in elucidate pathways of specialized metabolism. To promote the production of specialized metabolites in tomato fruit, this work expressed under a fruit ripening-specific promoter, E8, a bacterial AroG gene encoding a 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAHPS), which is feedback-insensitive to phenylalanine inhibition. DAHPS, the first enzyme of the shikimate pathway, links between the primary and specialized metabolism derived from aromatic amino acids. AroG expression influenced the levels of number of primary metabolites, such as shikimic acid and aromatic amino acids, as well as multiple volatile and non-volatile phenylpropanoids specialized metabolites and carotenoids. An organoleptic test, performed by trained panellists, suggested that the ripe AroG-expressing tomato fruits had a preferred floral aroma compare with fruits of the wild-type line. These results imply that fruit-specific manipulation of the conversion of primary to specialized metabolism is an attractive approach for improving fruit aroma and flavour qualities as well as discovering novel fruit-specialized metabolites.
- Subjects
TOMATO genetics; GENE expression; PHOSPHATES; SYNTHASES; SHIKIMATE 5-dehydrogenase; PLANT metabolites; BIOACTIVE compounds
- Publication
Journal of Experimental Botany, 2013, Vol 64, Issue 14, p4441
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/ert250