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- Title
Using fluorescent micro-particle image velocimetry to interrogate the endothelial surface layer in arterioles in vivo.
- Authors
Savery, Michele D.; Damiano, Edward R.
- Abstract
The existence of a hydrodynamically relevant endothelial surface layer (ESL) in capillaries and post-capillary venules is well established in the microcirculation of the mouse and other species. However, it is not known if the ESL is ubiquitous throughout the microcirculation. Since the ESL is likely to have broad implications for endothelial-cell function, and particularly for endothelial-cell mechanotransduction, it is important to establish the extent of its expression in arterioles. To determine whether a hydrodynamically relevant ESL exists in arterioles in vivo we apply microviscometric analysis to data obtained using micro-particle image velocimetry (µ-PIV) in cremaster muscle arterioles of wild-type mice. Data acquisition is triggered with the EKG at the same point in each cardiac cycle. Using µ-PIV data of the instantaneous speeds and radial positions of fluorescently labeled microspheres (∼0.5 µm diameter) in the midsaggital plane of the vessel, the velocity profile of the fluid is inferred and used to estimate the hydrodynamically relevant thickness of the ESL. Results will be presented for arterioles 20-50 µm in diameter before and after enzyme treatment to degrade the ESL.
- Subjects
VELOCIMETRY; ENDOTHELIUM; CAPILLARIES; MICROCIRCULATION; ARTERIES; BODY fluid flow; ENZYMES
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA484
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.5.a484-b