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- Title
Hypoxia induces the activation of human hepatic stellate cells LX-2 through TGF-β signaling pathway
- Authors
Shi, Yue-Feng; Fong, Chi-Chun; Zhang, Qi; Cheung, Pik-Yuen; Tzang, Chi-Hung; Wu, Rudolf S.S.; Yang, Mengsu
- Abstract
Abstract: Hypoxia is a common environmental stress factor and is also associated with various physiological and pathological conditions such as fibrogenesis. The activation of hepatic stellate cells (HSCs) is the key event in the liver fibrogenesis. In this study, the behavior of human HSCs LX-2 in low oxygen tension (1% O2) was analyzed. Upon hypoxia, the expression of HIF-1α and VEGF gene was induced. The result of Western blotting showed that the expression of α-SMA was increased by hypoxic stimulation. Furthermore, the expression of MMP-2 and TIMP-1 genes was increased. Hypoxia also elevated the protein expression of the collagen type I in LX-2 cells. The analysis of TGF-β/Smad signaling pathway showed that hypoxia potentiated the expression of TGF-β1 and the phosphorylation status of Smad2. Gene expression profiles of LX-2 cells induced by hypoxia were obtained by using cDNA microarray technique.
- Subjects
HYPOXEMIA; LIVER cells; GENE expression; COLLAGEN
- Publication
FEBS Letters, 2007, Vol 581, Issue 2, p203
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.12.010