We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Functional analysis of PU.1 domains in monocyte-specific gene regulation
- Authors
Nishiyama, Chiharu; Nishiyama, Makoto; Ito, Tomonobu; Masaki, Shigehiro; Masuoka, Nobutaka; Yamane, Hisakazu; Kitamura, Toshio; Ogawa, Hideoki; Okumura, Ko
- Abstract
The Ets family transcription factor PU.1 is required for the development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Recently we found that overproduction of PU.1 in mouse bone marrow-derived mast cell progenitors induced the expression of monocyte-specific genes. This prompted us to analyze the functions of each domain of PU.1 in monocyte-specific gene expression, using transfection of mast cell progenitors with a series of retrovirus vectors for overexpression of various truncation mutants. Both the acidic region and the Ets domain of PU.1 were required for expression of monocyte-specific genes, and for enhanced interleukin-6 production in response to lipopolysaccharide. The Gln-rich region was suggested to be involved in expression of both MHC class II and F4/80. On the other hand, when PU.1 protein lacking the PEST domain was produced in the progenitor cells, expression of monocyte-specific genes was substantially enhanced, suggesting that the PEST domain plays a negative role in monocyte-specific gene expression.
- Subjects
LYMPHOID tissue; GENETIC regulation; MAST cells; RETROVIRUSES
- Publication
FEBS Letters, 2004, Vol 561, Issue 1-3, p63
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00116-4