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- Title
Calcium-dependent conformational changes of membrane-bound Ebola fusion peptide drive vesicle fusion
- Authors
Suárez, Tatiana; Gómara, Marıa J.; Goñi, Félix M.; Mingarro, Ismael; Muga, Arturo; Pérez-Payá, Enrique; Nieva, José L.
- Abstract
The fusogenic subdomain of the Ebola virus envelope glycoprotein is an internal sequence located ca. 20 residues downstream the N-terminus of the glycoprotein transmembrane subunit. Partitioning of the Ebola fusion peptide into membranes containing phosphatidylinositol in the absence of Ca2+ stabilizes an α-helical conformation, and gives rise to vesicle efflux but not vesicle fusion. In the presence of millimolar Ca2+ the membrane-bound peptide adopts an extended β-structure, and induces inter-vesicle mixing of lipids. The peptide conformational polymorphism may be related to the flexibility of the virus–cell intermembrane fusogenic complex.
- Subjects
GLYCOPROTEINS; MEMBRANE fusion; PEPTIDES
- Publication
FEBS Letters, 2003, Vol 535, Issue 1-3, p23
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03847-4