We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Washing soil contaminated with crude oil using biosurfactant of halotolerant Bacillus cereus, grown under different conditions.
- Authors
Gracida, Jorge; Corona, Ana; Amaro, Aldo; Abreu, Arturo; Zamudio, Evelyn
- Abstract
The present work aims to report to removal of crude oil through soil washing with a biosurfactant (BS) produced by Bacillus cereus. To obtain the biosurfactant, different carbon sources were evaluated, as well as different NaCl concentrations. The strain of Bacillus cereus was isolated from saline soil. The carbon sources used in culture media were crude oil, glucose, and glycerol. Later the effect of NaCl concentration over surface tension (ST) was evaluated using the carbon sources (CS) separated. The washing capacity of the culture supernatant (free the cells) was tested in two contaminated soils with oil (3,200 and 10,000 ppm) and was compared with the synthetic surfactant polyoxyethylene (20) sorbitan monolaurate (Tween 20) and water as a blank. It was possible to obtain a decrease in surface tension value of 41.4 ± 1.3 mNm− 1, and an emulsification index of 78.4% using crude oil as CS at 0.6 and 2.0% (vv− 1) of NaCl in the culture media. Removal of oil of approximately 60% in the contaminated soil (3,200 ppm) was achieved using the supernatant that contained the BS, 8% more than the synthetic surfactant (Tween 20), while in the soil with 10,000 ppm, the removal was 64.5% and 41.0% for BS and Tween 20 respectively. Bacillus cereus was checked to have the capacity to produce biosurfactants when some of its parameters were modified in the culture medium. Biosurfactant generated also demonstrated its efficiency in hydrocarbon removal.
- Subjects
BIOSURFACTANTS; BACILLUS cereus; PETROLEUM; SOIL washing; SURFACE tension; SOIL pollution; SOILS
- Publication
Biologia, 2023, Vol 78, Issue 8, p2195
- ISSN
0006-3088
- Publication type
Article
- DOI
10.1007/s11756-023-01350-5