We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Analysis of <italic>PLA2R1</italic> and <italic>HLA</italic>-<italic>DQA1</italic> sequence variants in Japanese patients with idiopathic and secondary membranous nephropathy.
- Authors
Kaga, Hajime; Komatsuda, Atsushi; Omokawa, Ayumi; Okuyama, Shin; Mori, Kensuke; Wakui, Hideki; Takahashi, Naoto
- Abstract
Background: Several recent studies in patients with idiopathic membranous nephropathy (iMN) from Western and Asian counties showed that some single nucleotide polymorphisms (SNPs) within the <italic>PLA2R1</italic> and <italic>HLA</italic>-<italic>DQA1</italic> genes are significantly associated with iMN. However, there is only 1 report on analysis of <italic>PLA2R1</italic> and <italic>HLA</italic> regions in Japanese patients with iMN.Methods: A total of 58 patients with iMN, 26 patients with secondary MN (sMN), and 50 patients with other diseases were enrolled. All patients were Japanese. We selected 6 SNPs within <italic>PLA2R1</italic> and 1 SNP within <italic>HLA</italic>-<italic>DQA1</italic>, which were significantly associated with iMN in reported white European cohorts, and sequenced these exons using genomic DNA prepared from peripheral mononuclear cells from each patient. We then analyzed differences in <italic>PLA2R1</italic> and <italic>HLA</italic>-<italic>DQA1</italic> sequence variants among the 3 groups.Results: Genotypic and allelic frequency distributions for 3 out of 6 SNPs within <italic>PLA2R1</italic>, rs3749117, rs35771982, and rs2715918 were significantly different between the iMN and control groups. Allelic frequency distributions for SNP rs2187668 within <italic>HLA</italic>-<italic>DQA1</italic> were significantly different between the iMN and control groups. There were no correlations between <italic>PLA2R1</italic> and <italic>HLA</italic>-<italic>DQA1</italic> sequence variants and clinical parameters in patients with iMN. There were no significant differences in genotypic or allelic frequency distributions for examined SNPs between the sMN and control groups.Conclusions: There are some differences in <italic>PLA2R1</italic> SNP distributions between previously reported cohorts from other countries and our Japanese cohort of patients with iMN, while there is a significant association between SNP rs35771982 and iMN in most of reported cohorts.
- Subjects
JAPAN; KIDNEY diseases; SINGLE nucleotide polymorphisms; HLA class II antigens; EXONS (Genetics); EPIDEMIOLOGY
- Publication
Clinical & Experimental Nephrology, 2018, Vol 22, Issue 2, p275
- ISSN
1342-1751
- Publication type
Article
- DOI
10.1007/s10157-017-1471-0