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- Title
Coexistence of Psychrophilic, Mesophilic, and Thermophilic Sulfate-Reducing Bacteria in a Deep Subsurface Aquifer Associated with Coal-Bed Methane Production.
- Authors
Karnachuk, Olga V.; Panova, Inna A.; Rusanov, Igor I.; Schetinina, Lilia; Lepokurova, Olesya Y.; Domrocheva, Evgenia V.; Kadnikov, Vitaly V.; Avakyan, Marat R.; Lukina, Anstasia P.; Glukhova, Liubov B.; Pimenov, Nikolai V.; Ravin, Nikolai V.
- Abstract
The microbial community of subsurface environments remains understudied due to limited access to deep strata and aquifers. Coal-bed methane (CBM) production is associated with a large number of wells pumping water out of coal seams. CBM wells provide access to deep biotopes associated with coal-bed water. Temperature is one of the key constraints for the distribution and activity of subsurface microorganisms, including sulfate-reducing prokaryotes (SRP). The 16S rRNA gene amplicon sequencing coupled with in situ sulfate reduction rate (SRR) measurements with a radioactive tracer and cultivation at various temperatures revealed that the SRP community of the coal bed water of the Kuzbass coal basin is characterized by an overlapping mesophilic-psychrophilic boundary. The genus Desulfovibrio comprised a significant share of the SRP community. The D. psychrotolerans strain 1203, which has a growth optimum below 20 °C, dominated the cultivated SRP. SRR in coal bed water varied from 0.154 ± 0.07 to 2.04 ± 0.048 nmol S cm−3 day−1. Despite the ambient water temperature of ~ 10–20 °C, an active thermophilic SRP community occurred in the fracture water, which reduced sulfate with the rate of 0.159 ± 0.023 to 0.198 ± 0.007 nmol S cm−3 day−1 at 55 °C. A novel moderately thermophilic "Desulforudis audaxviator"-clade SRP has been isolated in pure culture from the coal-bed water.
- Subjects
SULFATE-reducing bacteria; THERMOPHILIC bacteria; GAS wells; COALBED methane; RADIOACTIVE tracers; COAL basins
- Publication
Microbial Ecology, 2023, Vol 86, Issue 3, p1934
- ISSN
0095-3628
- Publication type
Article
- DOI
10.1007/s00248-023-02196-9