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- Title
Expressions of nuclear factor-kappa B p50 and p65 and their significance in the up-regulation of intercellular cell adhesion molecule-1 mRNA in the nasal mucosa of allergic rhinitis patients.
- Authors
Wang, Shi; Ma, Feng; Zhao, Jian
- Abstract
The purpose of the study was to investigate expressions of nuclear factor-kappa B (NF-κB) and intercellular cell adhesion molecule-1 mRNA (ICAM-1 mRNA) in the nasal mucosa of allergic rhinitis (AR) patients. Expressions of NF-κB and ICAM-1 mRNA were studied using immunohistochemistry and reverse transcription-PCR (RT-PCR) in AR tissues and corresponding normal nasal mucosa. The correlation between NF-κB and ICAM-1 mRNA was studied using linear correlation analysis. The results of immunohistochemistry showed that expression of NF-κB was significantly up-regulated in the nasal mucosa of AR compared with that in normal tissue ( P < 0.01), over-expression of NF-κB p50 was found in the cytoplasm and nucleus ( P < 0.01), and NF-κB p65 was mainly expressed in the cytoplasm ( P < 0.01). ICAM-1 mRNA was strongly expressed in the nasal mucosa of AR compared with that in normal tissue as shown by RT-PCR ( P < 0.01). Up-regulation of ICAM-1 mRNA was significantly correlated with over-expressions of NF-κB p50 and NF-κB p65 ( r = 0.8995, P < 0.01; r = 0.7601, P < 0.01). In conclusion, NF-κB plays a key role in AR. Excessively activated NF-κB promotes the transcription of ICAM-1 mRNA. ICAM-1 is related to the pathogenesis and development of AR.
- Subjects
NF-kappa B; CELL adhesion molecules; ALLERGIC rhinitis; MESSENGER RNA; NASAL mucosa; PATIENTS
- Publication
European Archives of Oto-Rhino-Laryngology, 2013, Vol 270, Issue 4, p1329
- ISSN
0937-4477
- Publication type
Article
- DOI
10.1007/s00405-012-2136-y