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- Title
IN VITRO CULTURE ESTABLISHMENT AND CALLUS PROLIFERATION IN SIMAROUBA GLAUCA L.
- Authors
Dwivedi, Kuldip; Sharma, Rita; Bajpai, S. P.; Shukla, Nidhi
- Abstract
Simarouba glauca, a multipurpose, evergreen tree capable of withstanding diverse agro climatic conditions is indigenous to South Florida, West Indies and Brazil. Now it is cultivated in few regions of India like Maharashtra, Orissa, Rajasthan, Tamil Nadu, Karnataka and Andhra Pradesh. It has oil composition similar to popular oil feedstock such as Jatropha curcas that are being used to produce biodiesel. Poor seed germination, delayed flowering and failure of vegetative cutting paved way for the necessity to develop micro propagation techniques of this plant through organogenesis and callus cultures. An efficient regeneration protocol of Simarouba glauca was undertaken from nodal segment, shoot tip, leaf tips and leaf petiole explants to see the possibility of developing faster and reliable growth. Callus was induced under various medium compositions. The maximum callus induction frequency is reported with the MS basal media supplemented with 2.5mg/L 2, 4-D (CIM-4) in shoot tip explants and required least days for culture establishment. Nodal segments under similar medium concentration also resulted in better establishment of cultures as compared to the leaf segments whereas BAP and NAA along with MS basal medium were helpful in callus induction but failed to initiate further regeneration. Thus, results from present investigation clearly indicated the possibility of multiplying Simarouba glauca through micro propagation using above medium compositions and would help in increasing its population rapidly in India.
- Subjects
CALLUS; SIMAROUBACEAE; PLANT micropropagation; MORPHOGENESIS; GERMINATION
- Publication
Plant Archives (09725210), 2020, Vol 20, Issue 2, p2497
- ISSN
0972-5210
- Publication type
Article