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- Title
Imaging the cell surface: Argon sputtering to expose inner cell structures.
- Authors
Gelsomina De Stasio; Bradley H. Frazer; Marco Girasole; Lisa M. Wiese; Ewa K. Krasnowska; Giulia Greco; Annalucia Serafino; Tiziana Parasassi
- Abstract
Established microscopies such as Scanning Electron Microscopy (SEM) and more recent developments such as Atomic Force Microscopy (AFM) and X-ray Photo-Electron Emission spectroMicroscopy (X-PEEM) can only image the sample surface. We present an argon sputtering method able to progressively expose inner cell structures without apparent damage. By varying the sputtering time, the structure of cell cytoskeleton, vesicles, mitochondria, nuclear membrane, and nucleoli can be imaged. We compared images obtained with confocal fluorescence microscopy, transmission electron microscopy (TEM), SEM, and X-PEEM on similar samples after argon sputtering, then confirmed the similarity of reference intracellular structures, including cytoskeleton fibers, cell-cell and cell-substrate adhesion structures, and secretory vesicles. We conclude that the sputtering method is a new valuable tool for surface sensitive microscopies. Microsc. Res. Tech. 63:115121, 2004. © 2004 Wiley-Liss, Inc.
- Subjects
CELLS; MITOCHONDRIA; NUCLEAR membranes; SCANNING electron microscopy
- Publication
Microscopy Research & Technique, 2004, Vol 63, Issue 2, p115
- ISSN
1059-910X
- Publication type
Article
- DOI
10.1002/jemt.20019