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- Title
The isolation and mapping of 19 tetranucleotide microsatellite markers in the chicken.
- Authors
McConnell, S. K. J.; Dawson, D. A.; Wardle, A.; Burke, T.
- Abstract
Microsatellites consisting of tetranucleotide repeats are more easily, and consequently efficiently, scored than loci consisting ofdinucleotides. However, they are much less fre-quent in the genome. A hybridisation selection protocol was therefore employed to generate a chicken genomic library enriched for inserts containing the tetranucleotide repeat motif (TTTC)n. Forty-five new microsatellite sequences were isolated that mainly consisted of perfect repeats of the tetranucleotide (TTTC) motif. Nineteen markers were mapped in one or both of the East Lansing and Compton international chicken reference populations.
- Subjects
MICROSATELLITE repeats; CHICKENS; NUCLEOTIDE sequence; GENE libraries; LINKAGE (Genetics); GENE mapping
- Publication
Animal Genetics, 1999, Vol 30, Issue 3, p183
- ISSN
0268-9146
- Publication type
Article
- DOI
10.1046/j.1365-2052.1999.00454.x