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- Title
Comparing the genetic typing methods for effective surveillance and rabies control in Georgia.
- Authors
Condori, Rene E.; Kartskhia, Natia; Avaliani, Lasha; Donduashvili, Marina; Elbakidze, Tinatin; Kapanadze, Ana; Pieracci, Emily G.; Maghlakelidze, Giorgi; Wadhwa, Ashutosh; Morgan, Clint N.; Reynolds, Mary; Yu Li; Ninidze, Lena
- Abstract
A full nucleoprotein gene sequencing of 68 isolates collected from passive rabies surveillance system in Georgia between 2015 and 2016 identified two distinct dog rabies phylogroups, GEO_V1 and GEO_V2, which both belonged to the cosmopolitan dog clade. GEO_V1 was found throughout the country and was further divided into four sub-phylogroups that overlapped geographically; GEO_V2 was found in the southeast region and was closely related to dog rabies in Azerbaijan. A sequence analysis of the full N gene, partial nucleoprotein gene of N-terminal and C-terminal, and the amplicon sequences of pan-lyssavirus RT-qPCR LN34 showed that all four sequencing approaches provided clear genetic typing results of canine rabies and could further differentiate GEO_V1 and GEO_V2. The phylogenetic analysis results vary and were affected by the length of the sequences used. Amplicon sequencing of the LN34 assay positive samples provided a rapid and cost-effective method for rabies genetic typing, which is important for improving rabies surveillance and canine rabies eradication globally.
- Subjects
GEORGIA; AZERBAIJAN; RABIES; SEQUENCE analysis
- Publication
Frontiers in Microbiology, 2023, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2023.1243510