We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood.
- Authors
Benzine, Jason W.; Brown, Kerry M.; Agans, Krystle N.; Godiska, Ronald; Mire, Chad E.; Gowda, Krishne; Converse, Brandon; Geisbert, Thomas W.; Mead, David A.; Chander, Yogesh
- Abstract
A molecular diagnostic method for robust detection of Ebola virus (EBOV) at the point of care (POC) directly from blood samples is described. This assay is based on reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) of the glycoprotein gene of EBOV. Complete reaction formulations were lyophilized in 0.2-mL polymerase chain reaction tubes. RT-LAMP reactions were performed on a battery-operated isothermal instrument. Limit of detection of this RT-LAMP assay was 2.8 × 10² plaque-forming units (PFU)/test and 1 × 10³ PFU/test within 40 minutes for EBOV-Kikwit and EBOV-Makona, respectively. This assay was found to be specific for the detection of EBOV, as no nonspecific amplification was detected in blood samples spiked with closely related viruses and other pathogens. These results showed that this diagnostic test can be used at the point of care for rapid and specific detection of EBOV directly from blood with high sensitivity within 40 minutes.
- Subjects
EBOLA virus disease; MOLECULAR diagnosis; POINT-of-care testing; BLOOD sampling; REVERSE transcriptase polymerase chain reaction; GENE amplification; DIAGNOSIS
- Publication
Journal of Infectious Diseases, 2016, Vol 214, pS234
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jiw330