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- Title
Screening of astaxanthin-overproducing Xanthophyllomyces dendrorhous strains via iterative ARTP mutagenesis and cell sorting by flow cytometry.
- Authors
Ding, Ruirui; Huang, Ruilin; Su, Hang; Li, Jiawen; Li, Fuli; Wang, Shi'an
- Abstract
Aims The astaxanthin-producing yeast Xanthophyllomyces dendrorhous is widely used in aquaculture. Due to the production of carotenoid, this yeast shows visible color; however, high-throughput approaches for identification of astaxanthin-overproducing strains remain rare. Methods and results This study verified an effective approach to identify astaxanthin-overproducing mutants of X. dendrorhous by flow cytometry (FCM) and cell sorting. First, the mutant libraries were generated by atmospheric and room-temperature plasma (ARTP) mutagenesis. Second, a highly direct correlation between the concentrations of intracellular astaxanthin and the levels of emitting fluorescence was constructed by testing a variety of astaxanthin-contained populations via FCM and cell sorting. Third, iterative cell sorting efficiently improves the identification of astaxanthin-overproducing strains. Finally, two mutants producing 4.96 mg astaxanthin g−1 DCW (dry cell weight) and 5.30 mg astaxanthin g−1 DCW were obtained, which were 25.3% and 33.8% higher than that of the original strain, respectively. Conclusions This study demonstrated that iterative ARTP mutagenesis along with cell sorting by FCM is effective for identifying astaxanthin-overproduction strains.
- Subjects
ASTAXANTHIN; FLOW cytometry; MUTAGENESIS
- Publication
Journal of Applied Microbiology, 2024, Vol 135, Issue 2, p1
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1093/jambio/lxae020