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- Title
Down-regulation of miR-27a might reverse multidrug resistance of esophageal squamous cell carcinoma.
- Authors
Hongwei Zhang; Mengbin Li; Yu Han; Liu Hong; Taiqian Gong; Li Sun; Xiushan Zheng; Zhang, Hongwei; Li, Mengbin; Han, Yu; Hong, Liu; Gong, Taiqian; Sun, Li; Zheng, Xiushan
- Abstract
<bold>Background: </bold>So far, the miRNAs involved in multidrug resistance of esophageal cancer have not been reported.<bold>Aims and Methods: </bold>Here we have firstly investigated the roles of miR-27a in multidrug resistance of esophageal squamous cell carcinoma using MTT assay, flow cytometry assay, and reporter gene assay, etc.<bold>Results: </bold>Down-regulation of miR-27a could confer sensitivity of both P-glycoprotein-related and P-glycoprotein-non-related drugs on esophageal cancer cells, and might promote ADR-induced apoptosis, accompanied by increased accumulation and decreased releasing amount of ADR. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein, Bcl-2, and the transcription of the multidrug resistance gene 1, but up-regulate the expression of Bax.<bold>Conclusions: </bold>MiR-27a might play important roles in multidrug resistance of esophageal cancer. The further study of the biological functions of miR-27a might be helpful for developing possible strategies to treat esophageal cancer.
- Subjects
MULTIDRUG resistance; APOPTOSIS; ESOPHAGEAL cancer; CANCER cells; GENE transfection; POLYMERASE chain reaction
- Publication
Digestive Diseases & Sciences, 2010, Vol 55, Issue 9, p2545
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-009-1051-6