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- Title
Prevalence, genetic diversity and phylogenetic analysis of PRRSV in Mexico.
- Authors
Escalante-Sansores, A.R.; Salazar-Bautista, E.; Martínez-Gomez, M. A.; Martínez-Sosa, X.E.; Garcia-Lopez, D.
- Abstract
Porcine Reproductive and Respiratory Syndrome (PRRS) is a highly significant disease due to the economic losses it causes globally. PRRS affects pigs of all ages, primarily causing reproductive failure in sows and respiratory disease in piglets. The etiological agent, PRRS virus (PRRSV), belongs to the order Nidovirales, family Arteriviridae, genus Porartevirus, with a positive-sense single-stranded RNA genome of 15 kb. The glycoprotein GP5 is encoded in the ORF5 gene, whose sequence is commonly used to establish phylogenetic relationships and classify PRRSV isolates. During routine monitoring of various swine production units in Mexico, a total of 5,840 samples, mainly serum/whole blood, oral fluids, and lung tissue, were analyzed for PRRSV diagnosis using real-time RT-PCR, resulting in an average positivity of 18.51 %. Positive samples were selected for Sanger sequencing of the ORF5 gene. The obtained sequences were aligned, and a maximum likelihood phylogenetic tree was constructed. The circulating PRRSV strains in Mexico were found to belong to lineages 1 (57 %) and 5 (43 %). The predominant RFLP pattern within lineage 1 was 1-7-4 type, while in lineage 5, 2-5-2 type. These findings shed light on the genetic diversity of PRRSV circulating in Mexico and provide valuable insights for the surveillance and control of the disease.
- Publication
Veterinaria México OA, 2024, Vol 11, p66
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1305