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- Title
PRDM16-DT is a novel lncRNA that regulates astrocyte function in Alzheimer's disease.
- Authors
Schröder, Sophie; Fuchs, Ulrike; Gisa, Verena; Pena, Tonatiuh; Krüger, Dennis M.; Hempel, Nina; Burkhardt, Susanne; Salinas, Gabriela; Schütz, Anna-Lena; Delalle, Ivana; Sananbenesi, Farahnaz; Fischer, Andre
- Abstract
Astrocytes provide crucial support for neurons, contributing to synaptogenesis, synaptic maintenance, and neurotransmitter recycling. Under pathological conditions, deregulation of astrocytes contributes to neurodegenerative diseases such as Alzheimer's disease (AD). While most research in this field has focused on protein-coding genes, non-coding RNAs, particularly long non-coding RNAs (lncRNAs), have emerged as significant regulatory molecules. In this study, we identified the lncRNA PRDM16-DT as highly enriched in the human brain, where it is almost exclusively expressed in astrocytes. PRDM16-DT and its murine homolog, Prdm16os, are downregulated in the brains of AD patients and in AD models. In line with this, knockdown of PRDM16-DT and Prdm16os revealed its critical role in maintaining astrocyte homeostasis and supporting neuronal function by regulating genes essential for glutamate uptake, lactate release, and neuronal spine density through interactions with the RE1-Silencing Transcription factor (Rest) and Polycomb Repressive Complex 2 (PRC2). Notably, CRISPR-mediated overexpression of Prdm16os mitigated functional deficits in astrocytes induced by stimuli linked to AD pathogenesis. These findings underscore the importance of PRDM16-DT in astrocyte function and its potential as a novel therapeutic target for neurodegenerative disorders characterized by astrocyte dysfunction.
- Subjects
ALZHEIMER'S disease; LINCRNA; TRANSCRIPTION factors; NON-coding RNA; NEURODEGENERATION
- Publication
Acta Neuropathologica, 2024, Vol 148, Issue 1, p1
- ISSN
0001-6322
- Publication type
Article
- DOI
10.1007/s00401-024-02787-x