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- Title
Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4.
- Authors
Colic, Miodrag; Jandric, Dusan; Stojic-Vukanic, Zorica; Antic-Stankovic, Jelena; Popovic, Petar; Vasilijic, Saša; Milosavljevic, Petar; Balint, Bela
- Abstract
Several laboratories have developed culture systems that allow the generation of large numbers of human dendritic cells (DC) from monocytes using granulocyte-macrophage colony stimulating factor (GM-CSF), and interleukin-4 (IL-4). In this work we provided evidence that GM-CSF (100 ng/ml) in combination with a low concentration of IL-4 (5 ng/ml) was efficient in the generation of immature, non-adherent, monocyte-derived DC as the same concentration of GM-CSF, and ten times higher concentration of IL-4 (50 ng/ml). This conclusion was based on the similar phenotype profile of DC. such as the expression of CD1a, CD80, CD86, and HLA-DR, down-regulation of CD 14, and the absence of CD83, as well as on their similar allostimulatory activity for T cells. A higher number of cells remained adherent in cultures with lower concentrations of IL-4 than in cultures with higher concentrations of the cytokine. However, most of these adherent cells down-regulated CD 14 and stimulated the proliferation of alloreactive T cells. In contrast, adherent cells cultivated with GM-CSF alone were predominantly macrophages, as judged by the expression of CD14 and the inefficiency to stimulate alloreactive T cells. DC generated in the presence of lower concentrations of IL-4 had higher proapoptotic potential for the Jurkat cell line than DC differentiated with higher concentrations of IL-4, suggesting their stronger cytotoxic, anti-tumor effect.
- Subjects
DENDRITIC cells; MONOCYTES; INTERLEUKIN-4; T cells; PHENOTYPES
- Publication
Vojnosanitetski Pregled: Military Medical & Pharmaceutical Journal of Serbia, 2003, Vol 60, Issue 5, p531
- ISSN
0042-8450
- Publication type
Article
- DOI
10.2298/VSP0305531C