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- Title
Construction and Characterization of a CTLA-4-Targeted scFv–Melittin Fusion Protein as a Potential Immunosuppressive Agent for Organ Transplant.
- Authors
Jin, Hailong; Li, Congran; Li, Ding; Cai, Ming; Li, Zhouli; Wang, Shuang; Hong, Xin; Shi, Bingyi
- Abstract
Immunotoxins with selective cytotoxicity are frequently used as therapeutic immunosuppressive agents in solid-organ transplantation because of their efficiency and high specificity. In this study, we present a new recombinant immunotoxin termed anti-CTLA-4-scFv–melittin prepared from Escherichia coli aimed at clearing activated T cells at the same time avoiding all-round decline in systematic immunity. This fusion protein is composed of anti-CTLA-4-scFv unit and melittin analog unit with properties of low immunogenicity and selective cytotoxicity to CTLA-4-positive T cells. In preliminary biological activity assays, our results confirmed the feasibility of activated T cell clearance strategy and there were significant differences in cell survival rates between CTLA-4-positive group and control group at all experimental concentrations of the immunotoxin. The selective cytotoxicity, low immunogenicity, and low production cost make it an attractive alternate to traditional immunosuppressants.
- Subjects
TRANSPLANTATION of organs, tissues, etc.; MELITTIN; CHIMERIC proteins; ESCHERICHIA coli; IMMUNOSUPPRESSIVE agents; ANTIBODY-toxin conjugates
- Publication
Cell Biochemistry & Biophysics, 2013, Vol 67, Issue 3, p1067
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-013-9605-9