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- Title
PADI4 mediates autophagy and participates in the role of ganoderic acid A monomers in delaying the senescence of Alzheimer's cells through the Akt/mTOR pathway.
- Authors
Shen, Shuhua; Wang, Xiaoming; Lv, Hang; Shi, Yuan; Xiao, Luwei
- Abstract
The effects of PADI4 and GAA on the senescence of Alzheimer's cells were explored in the present work. HT22 cells were treated with Aβ25-35 to establish an Alzheimer's model and were then treated with different concentrations of GAA and transfected with a siPADI4 lentiviral vector. GAA could reverse the effects of Aβ25-35 on inhibiting cell viability and promoting apoptosis and senescence. siPADI4 reduced Aβ25-35-induced cell viability and upregulated Aβ25-35-induced cell apoptosis and senescence, as well as partially reversed the effect of GAA on cells, and these results were confirmed by detecting the expressions of senescence- and apoptosis-related proteins. In addition, siPADI4 was found to promote the phosphorylation of Akt and mTOR, which was partially reversed by GAA. In conclusion, PADI4 mediates autophagy and participates in the role of GAA monomers in delaying the senescence of Alzheimer's cells through the Akt/mTOR pathway.
- Subjects
ALZHEIMER'S disease; AGING; AUTOPHAGY; CELLULAR aging; MONOMERS; CELL survival
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 8, p1818
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbab054