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- Title
Tumour necrosis factor-alpha-induced protein 8 ( TNFAIP8) expression associated with cell survival and death in cancer cell lines infected with canine distemper virus.
- Authors
Garcia, J. A.; Ferreira, H. L.; Vieira, F. V.; Gameiro, R.; Andrade, A. L.; Eugênio, F. R.; Flores, E. F.; Cardoso, T. C.
- Abstract
Oncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus ( CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine-derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine-derived adenofibrosarcoma cell line were tested regarding to their susceptibility to CDV infection, cell proliferation, apoptosis, mitochondrial membrane potential and expression of tumour necrosis factor-alpha-induced protein 8 ( TNFAIP8). CDV replication-induced cytopathic effect, decrease of cell proliferation rates, and >45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDVM gene expression were positively correlated in all cell lines. In addition, mitochondrial membrane depolarization was associated with increase in virus titres ( p < 0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV-induced cancer therapy.
- Subjects
CANINE distemper virus; PARAMYXOVIRUSES; CANCER treatment; APOPTOSIS; CELL proliferation
- Publication
Veterinary & Comparative Oncology, 2017, Vol 15, Issue 2, p336
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/vco.12168