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- Title
Highly parallel dispensing of chemical and biological reagents.
- Authors
de Heij, Bas; Daub, Martina; Gutmann, Oliver; Niekrawietz, Remigius; Sandmaier, Hermann; Zengerle, Roland
- Abstract
We present a technology for the highly parallel dispensing of a multitude of reagents. It allows one to dispense up to 96 different reagents simultaneously in a fixed array, in a volume range of 100 pL up to several nL. The pitch of the dispensed droplets can be as small as 500 µm. All channels are fired simultaneously, giving an unprecedented throughput. The system was originally developed for the high-throughput fabrication of microarrays, but can easily be adopted for other applications such as highly parallel filling of nanotiterplates. Based on our standard configuration we achieved droplets with 125-µm in-flight diameter (1.2 nL) with a CV of <1%.
- Subjects
BIOLOGICAL reagents; BIOLOGICAL assay; CHEMICAL reagents; DNA microarrays; ELECTRON-hole droplets; CHEMICALS
- Publication
Analytical & Bioanalytical Chemistry, 2004, Vol 378, Issue 1, p119
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-003-2294-2