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- Title
Analysis of serotonin release from single neuron soma using capillary electrophoresis and laser-induced fluorescence with a pulsed deep-UV NeCu laser.
- Authors
Miao, Hai; Rubakhin, Stanislav S.; Sweedler, Jonathan V.
- Abstract
The use of capillary electrophoresis (CE) with laser-induced fluorescence excited by ultraviolet (UV) lasers in the range 200–300 nm has been restricted by the available wavelengths and expense of UV lasers. The integration of a NeCu deep UV laser operating at 248.6 nm with a single channel CE system with post-column sheath flow detection allows detection limits for serotonin and tryptophan of 3.9×10-8 M and 4.5×10-8 M respectively. Single cell analysis of serotonergic metacerebral cells from the sea slug Aplysia californica yields a value of 800±85 fmol of serotonin in each cell soma. For the first time, serotonin is directly detected in electrically stimulated release from single metacerebral cell soma, with approximately 4% of the serotonin contained in the soma released from a semi-intact preparation with a 2 min electrical stimulation.
- Subjects
SEROTONIN; CAPILLARY electrophoresis; LASERS; ELECTRIC stimulation; LASER photochemistry; CELLS
- Publication
Analytical & Bioanalytical Chemistry, 2003, Vol 377, Issue 6, p1007
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-003-2191-8