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- Title
Characterization of a partial amphiploid between Triticum aestivum cv. Chinese Spring and Thinopyrum intermedium ssp. trichophorum.
- Authors
Zu-Jun Yang; Guang-Rong Li; Zhi-Jian Chang; Jian-Ping Zhou; Zheng-Long Ren
- Abstract
A partial amphiploid, TE-3, between Triticum aestivum cv. Chinese Spring (CS) and Thinopyrum intermedium ssp . trichophorum was characterized by cytological observation, genomic in situ hybridization (GISH), seed storage protein electrophoresis and disease resistance screening. The TE-3 plants were deeply covered with pubescence, which is characteristic of the Th. intermedium ssp . trichophorum parent. Feulgen staining of the somatic metaphases revealed that the chromosome number varied from 52 to 56. TE-3 pollen mother cells (PMCs) regularly showed two to four univalents and 25 to 27 bivalents, indicating a degree of cytological instability. Giemsa-C banding showed that the Thinopyrum chromosomes in TE-3 produced strong heterochromatin bands. GISH analysis suggested that the alien chromosomes in TE-3 consisted of eight St chromosomes, four Js chromosomes, and two J genome chromosomes, as well as two St-J translocation chromosomes. Seeds storage proteins separated by acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulphate – polyacrylamide gel electrophoresis (SDS-PAGE) showed that TE-3 expressed some of Th. intermedium ssp . trichophorum specific gliadin and glutenin bands. When inoculated with stripe rust and powdery mildew isolates, TE-3 expressed resistance derived from its Thinopyrum parent. It appears that TE-3 can be used as a donor source in wheat breeding programs to introduce novel variation for quality and disease resistance.
- Subjects
WHEAT; IN situ hybridization; GENOMICS; DISEASE resistance of plants; CYTOLOGY; ELECTROPHORESIS
- Publication
Euphytica, 2006, Vol 149, Issue 1/2, p11
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-005-9010-6