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- Title
Properties of bacterial cellulose developed from genetically stable FM833 bacterial strain for 30 generations via Kombucha tea.
- Authors
Zheng, Meixia; Chen, Meichun; Chen, Jianfu; Tan, Kok Bing; Xiao, Rongfeng; Chen, Yanping; Zhu, Yujing
- Abstract
The quality control of the bacterial cellulose (BC) production has been facing challenges due to the genetic stability of bacterial strains, which is often overlooked or underestimated. Thus, its commercialized application is limited. Therefore, a bacterial strain FM883 with a 16S rRNA sequence 97% identical to Komagataeibacter xylinus NBRC15237T was isolated from kombucha tea. Cultivation of FM883 was conducted in series to observe the morphology and BC yield among different generations from 1st generation to 30th generation. The results show that FM883 can efficiently produce BC in modified Hestrin-Schramn media at the 30th generation (163.5 ± 0.3 g/L), which was even higher than the 1st generation (157.6 ± 0.3 g/L). This indicated that FM883 has strong genetic stability. Furthermore, several characterizations of the BC indicated that the quality of the BC biosynthesized by FM883 was good with excellent thermostability. With such strong genetic stability, and properties, as well as sufficiently high consistency and high yield, BC biosynthesized by FM883 had demonstrated excellent production quality control, which is desired for commercial manufacturing.
- Subjects
KOMBUCHA tea; PRODUCTION control; QUALITY control
- Publication
Cellulose, 2022, Vol 29, Issue 16, p8509
- ISSN
0969-0239
- Publication type
Article
- DOI
10.1007/s10570-022-04799-w