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- Title
Enantioselective production of levofloxacin by immobilized porcine liver esterase.
- Authors
Lee, Sang-Yoon; Min, Byung-Hyuk; Hwang, Sung-Ho; Koo, Yoon-Mo; Lee, Choul-Kyun; Song, Seong-Won; Oh, Sun-Young; Lim, Sang-Min; Kim, Sang-Lin; Kim, Dong-II
- Abstract
Porcine liver esterase, which cleaves ofloxacin butyl ester enantioselectively to levofloxacin, was successfully immobilized in calcium alginate and polyacrylamide gel. Immobilized esterase in 5% (w/v) calcium alginate exhibited 58% immobilization efficiency and could be reused five times without severe loss of enzyme activity. On the other hand, entrapped esterase in polyacrylamide gel, composed of 20% of total monomer and 8.3% of cross-linking agent, could be reused 10 times, and 51% of enzyme activity remained after the 10th batch without decrease of enantioselectivity. Compared with entrapped methods, significant reduction of enzyme activity was found in the case of physical adsorption on to QAE-Sephadex.
- Subjects
ENANTIOSELECTIVE catalysis; HETEROGENEOUS catalysis; BILIARY tract; ENZYMES; CALCIUM; POLYMERS
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 13, p1033
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1010554118301